This product is a virus-like Paricle (VLP) formed by self-assembly after the capsid proteins L1 and L2 of different types of Human Papilloma Virus (HPV) are jointly expressed in 293T cells. At the same time, the nucleic acid sequence containing the reporter gene is wrapped inside the pseudovirus. This pseudovirus has a structure highly similar to that of a live virus, possesses the vitality of single-round infection cells, can express reporter genes, and has good safety.

Product Application

1）It is used for the titer test of neutralizing antibodies against HPV in serum.

2) Detection of monoclonal antibody neutralizing antibody titers.

Detection principle

Neutralizing antibodies can specifically recognize the antigen-neutralizing sites on the surface of viruses, prevent viruses from invading cells and multiplying, and protect the body from their harm, thus having a true antiviral effect. HPV neutralizing antibody pseudovirus contains the neutralizing antigen of the virus and has the vitality of infecting cells. Neutralizing antibodies can recognize HPV pseudoviruses and undergo neutralization reactions, reducing the infection ability of HPV pseudoviruses and leading to weakened expression levels of the reporter genes carried (such as green fluorescent protein zsGreen, etc.). The inhibition percentage of the sample to be tested can be calculated by scanning and counting the number of fluorescent spots in each well with an Elispot reader and comparing the values of the sample well with those of the pseudovirus control group. Calculate the reciprocal of the dilution factor of the antibody when 50% of the pseudovirus is inhibited, which is the neutralizing antibody titer.

HPV假病毒感染293T细胞72 h后检测荧光素酶表达